1QC0

CRYSTAL STRUCTURE OF A 19 BASE PAIR COPY CONTROL RELATED RNA DUPLEX

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	100
Detector technology	CCD
Collection date	1997-11-15
Detector	ADSC QUANTUM 4
Spacegroup name	H 3 2
Unit cell lengths	43.682, 43.682, 447.021
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.000 - 1.550
R-factor	0.15 *
Rwork	0.150
R-free	0.21440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	A-RNA
RMSD bond length	0.010 *
RMSD bond angle	0.018
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	1.610
High resolution limit [Å]	1.550	1.550
Rmerge	0.052	0.452
Number of reflections	22779
<I/σ(I)>	26.9	2.7
Completeness [%]	91.5	95 *
Redundancy	3.6	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6.5	20 *	(NH4)2SO4, SODIUM CACODYLATE, MGCL2, ROP PROTEIN, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	Vapor diffusion *	6.5	20 *	(NH4)2SO4, SODIUM CACODYLATE, MGCL2, ROP PROTEIN, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	(NH4)2SO4
2	1	1	SODIUM CACODYLATE
3	1	1	MGCL2
4	1	1	ROP PROTEIN
5	1	2	(NH4)2SO4

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	RNA oligo	1.0 (mM)
2	1	drop	rop protein	1.0 (mM)
3	1	drop	ammonium sulfate	1.05 (M)
4	1	drop	sodium cacodylate	5 (mM)
5	1	drop		1 (mM)
6	1	drop	sodium azide	0.02 (%)
7	1	reservoir	ammonium sulfate	2.1 (M)