1QBQ
STRUCTURE OF RAT FARNESYL PROTEIN TRANSFERASE COMPLEXED WITH A CVIM PEPTIDE AND ALPHA-HYDROXYFARNESYLPHOSPHONIC ACID.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 95 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-10-06 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | P 61 |
| Unit cell lengths | 174.132, 174.132, 69.705 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 15.000 - 2.400 |
| R-factor | 0.218 * |
| Rwork | 0.218 |
| R-free | 0.29200 |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.500 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.061 | 0.371 |
| Total number of observations | 144714 * | |
| Number of reflections | 42739 | |
| <I/σ(I)> | 20 | |
| Completeness [%] | 90.0 | 57 |
| Redundancy | 3.3 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.7 * | 22 * | 7% PEG 4000, 0.1 M sodium acetate, pH 5.7, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris | 20 (mM) | |
| 2 | 1 | drop | dithiothreitol | 1 (mM) | |
| 3 | 1 | drop | 20 (mM) | ||
| 4 | 1 | drop | 0.010 (mM) | ||
| 5 | 1 | drop | protain | 25 (mg/ml) | |
| 6 | 1 | reservoir | PEG4000 | 7 (%) | |
| 7 | 1 | reservoir | sodium acetate | 0.1 (M) | |
| 8 | 1 | reservoir | dithiothreitol | 200 (mM) |
