1QBM

FAB E8B ANTIBODY, X-RAY STRUCTURE AT 2.37 ANGSTROMS RESOLUTION

Experimental procedure

Temperature [K]	291
Detector technology	AREA DETECTOR
Detector	SIEMENS
Spacegroup name	P 1 21 1
Unit cell lengths	44.960, 85.010, 63.330
Unit cell angles	90.00, 105.00, 90.00

Refinement procedure

Resolution	10.000 - 2.370
R-factor	0.194
Rwork	0.194
R-free	0.26900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2.26 ANGSTROM RESOLUTION FAB E8A
RMSD bond length	0.011 *
RMSD bond angle	28.300 *
Data reduction software	XENGEN
Data scaling software	XENGEN
Phasing software	X-PLOR (3.8)
Refinement software	X-PLOR (3.8)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]		2.470
High resolution limit [Å]	2.370	2.370
Rmerge	0.146 *	0.440 *
Number of reflections	17924
<I/σ(I)>	14	2
Completeness [%]	91.6	52.3
Redundancy	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	6.2 *		pH 6.4

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	sodium ammonium phosphate	0.05 (M)
2	1	1	PEG4000	20 (%(w/v))