1QBL

FAB E8 (FABE8A) X-RAY STRUCTURE AT 2.26 ANGSTROM RESOLUTION

Experimental procedure

Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	90
Detector technology	IMAGE PLATE
Collection date	1994-07-04
Detector	MARRESEARCH
Spacegroup name	P 1 21 1
Unit cell lengths	44.840, 82.330, 64.520
Unit cell angles	90.00, 104.10, 90.00

Refinement procedure

Resolution	10.000 - 2.260
R-factor	0.191 *
Rwork	0.192
R-free	0.27700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1.9 A RESOLUTION FAB J539
RMSD bond length	0.010
RMSD bond angle	28.400 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.8)
Refinement software	X-PLOR (3.8)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.360
High resolution limit [Å]	2.260	2.260
Rmerge	0.073 *
Number of reflections	21365
<I/σ(I)>	26.2	8
Completeness [%]	99.7	99.3 *
Redundancy	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6.2		0.05M SODIUM AMMONIUM PHOSPHATE BUFFER, PH 6.2, 25% (W/V) PEG 4000.

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	sodium ammonium	0.05 (M)
2	1	reservoir	PEG4000	20 (%(w/v))