1QBI
SOLUBLE QUINOPROTEIN GLUCOSE DEHYDROGENASE FROM ACINETOBACTER CALCOACETICUS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-05-20 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.148, 92.100, 84.910 |
| Unit cell angles | 90.00, 105.17, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.720 |
| R-factor | 0.166 |
| Rwork | 0.165 |
| R-free | 0.20800 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 2.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASES |
| Refinement software | X-PLOR (3.843) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.058 | 0.198 |
| Number of reflections | 83663 | |
| <I/σ(I)> | 18.3 | |
| Completeness [%] | 85.9 | 27.3 |
| Redundancy | 3.3 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 9.2 | 293 | PEG 6000, SODIUM CHLORIDE, CALCIUM CHLORIDE, TRIS, GLYCINE, pH 9.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 2 | 1 | drop | PQQ | 0.1 (mM) | |
| 3 | 1 | drop | PEG6000 | 8 (%(w/v)) | |
| 4 | 1 | drop | Tris-glycine | 50 (mM) | |
| 5 | 1 | reservoir | PEG6000 | 20-23 (%(w/v)) | |
| 6 | 1 | reservoir | 120 (mM) | ||
| 7 | 1 | reservoir | 3 (mM) | ||
| 8 | 1 | reservoir | Tris-glycine | 50 (mM) |
