1QB5
ESCHERICHIA COLI HEAT LABILE ENTEROTOXIN TYPE IIB B-PENTAMER
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 275 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 76.091, 67.573, 90.287 |
| Unit cell angles | 90.00, 113.15, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.900 |
| R-factor | 0.177 |
| Rwork | 0.177 |
| R-free | 0.23700 |
| RMSD bond length | 0.000 |
| RMSD bond angle | 0.010 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.101 | 0.335 |
| Number of reflections | 36737 | |
| <I/σ(I)> | 14 | 5.4 |
| Completeness [%] | 94.0 | 92 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | VAPOR DIFFUSION, SITTING DROP, pH 5.5 |
