1QB0
HUMAN CDC25B CATALYTIC DOMAIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | SIEMENS |
| Temperature [K] | 100 |
| Detector technology | AREA DETECTOR |
| Collection date | 1997-09-29 |
| Detector | SIEMENS HI-STAR |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.169, 71.097, 73.515 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.910 |
| R-factor | 0.1348 |
| R-free | 0.17800 * |
| Structure solution method | MIR |
| RMSD bond length | 0.030 |
| RMSD bond angle | 0.200 |
| Data reduction software | SADIE |
| Data scaling software | SAINT |
| Phasing software | XTAL (V. 3.0) |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.980 |
| High resolution limit [Å] | 1.910 | 1.910 |
| Rmerge | 0.042 | 0.140 |
| Total number of observations | 76415 * | |
| Number of reflections | 21239 | |
| <I/σ(I)> | 28.5 | 5.4 |
| Completeness [%] | 95.1 | 55.7 |
| Redundancy | 3.6 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 277 | 4 MICROLITERS PROTEIN (10 MG/ML IN 50 MM TRISHCL, 1% BME AT PH 9.0) MIXED WITH 4 MICROLITERS WELL BUFFER (1.8 (NH4)2SO4, 0.5 M NACL, 0.1 M TRISHCL, 0.25 BME) AT 4 DEG. C, VAPOR DIFFUSION, SITTING DROP, temperature 4.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 50 (mM) | |
| 3 | 1 | drop | beta-mercaptoethanol | 1 (%(v/v)) | |
| 4 | 1 | reservoir | ammonium sulfate | 1.8 (M) | |
| 5 | 1 | reservoir | 0.5 (M) | ||
| 6 | 1 | reservoir | Tris-HCl | 0.1 (M) | |
| 7 | 1 | reservoir | BME | 0.25 (%) |
