1QAY
TERNARY COMPLEX OF PSEUDOMONAS MEVALONII HMG-COA REDUCTASE WITH MEVALONATE AND NAD+
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 295 |
Detector technology | IMAGE PLATE |
Collection date | 1997-01-01 |
Detector | RIGAKU RAXIS II |
Spacegroup name | I 41 3 2 |
Unit cell lengths | 228.500, 228.500, 228.500 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 2.800 |
R-factor | 0.188 |
Rwork | 0.204 |
R-free | 0.26600 |
RMSD bond length | 0.013 |
RMSD bond angle | 1.700 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.080 | 0.290 |
Number of reflections | 23498 | |
<I/σ(I)> | 15 | |
Completeness [%] | 93.1 | 73.4 |
Redundancy | 3 | 1.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 8.2 * | VAPOR DIFFUSION, 295K, PH 6.7, 1.2M AMMONIUM SULFATE, 100MM ADA BUFFER |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | Tris-HCl | 10 (mM) | |
2 | 1 | drop | 400 (mM) | ||
3 | 1 | drop | glycerol | 10 (%) | |
4 | 1 | reservoir | ammonium sulfate | 1.2 (M) | |
5 | 1 | reservoir | sodium citrate | 75 (mM) | |
6 | 1 | reservoir | sodium ADA | 100 (mM) |