1QAP

QUINOLINIC ACID PHOSPHORIBOSYLTRANSFERASE WITH BOUND QUINOLINIC ACID

Experimental procedure

Detector technology	AREA DETECTOR
Collection date	1995
Detector	SIEMENS
Spacegroup name	P 41 21 2
Unit cell lengths	81.400, 81.400, 217.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 2.800
R-factor	0.186
Rwork	0.186
R-free	0.27400
RMSD bond length	0.010
Data reduction software	XENGEN
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.800 *
Rmerge	0.120 *
Number of reflections	17451
Completeness [%]	93.0 *
Redundancy	5.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8.1 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5-10 (mg/ml)
10	1	reservoir	HEPES	0.1 (M)
11	1	reservoir		0.2 (M)
2	1	drop	Tris-HCl	10 (mM)
3	1	drop		0.025 (%)
4	1	drop	dithiothreitol	1 (mM)
5	1	drop	QA	1 (mM)
6	1	drop	PEG400	12.5-15 (%)
7	1	drop	HEPES	0.05 (M)
8	1	drop		0.1 (M)
9	1	reservoir	PEG400	25-30 (%)