1QAP
QUINOLINIC ACID PHOSPHORIBOSYLTRANSFERASE WITH BOUND QUINOLINIC ACID
Experimental procedure
| Detector technology | AREA DETECTOR |
| Collection date | 1995 |
| Detector | SIEMENS |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 81.400, 81.400, 217.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.800 |
| R-factor | 0.186 |
| Rwork | 0.186 |
| R-free | 0.27400 |
| RMSD bond length | 0.010 |
| Data reduction software | XENGEN |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.800 * |
| Rmerge | 0.120 * |
| Number of reflections | 17451 |
| Completeness [%] | 93.0 * |
| Redundancy | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8.1 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5-10 (mg/ml) | |
| 10 | 1 | reservoir | HEPES | 0.1 (M) | |
| 11 | 1 | reservoir | 0.2 (M) | ||
| 2 | 1 | drop | Tris-HCl | 10 (mM) | |
| 3 | 1 | drop | 0.025 (%) | ||
| 4 | 1 | drop | dithiothreitol | 1 (mM) | |
| 5 | 1 | drop | QA | 1 (mM) | |
| 6 | 1 | drop | PEG400 | 12.5-15 (%) | |
| 7 | 1 | drop | HEPES | 0.05 (M) | |
| 8 | 1 | drop | 0.1 (M) | ||
| 9 | 1 | reservoir | PEG400 | 25-30 (%) |
