1QAI

CRYSTAL STRUCTURES OF THE N-TERMINAL FRAGMENT FROM MOLONEY MURINE LEUKEMIA VIRUS REVERSE TRANSCRIPTASE COMPLEXED WITH NUCLEIC ACID: FUNCTIONAL IMPLICATIONS FOR TEMPLATE-PRIMER BINDING TO THE FINGERS DOMAIN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	FUJI
Spacegroup name	P 1 21 1
Unit cell lengths	61.138, 38.432, 129.751
Unit cell angles	90.00, 100.58, 90.00

Refinement procedure

Resolution	10.000 - 2.300
R-factor	0.233
Rwork	0.233
R-free	0.29600
RMSD bond length	0.006
RMSD bond angle	1.320
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	10.000	2.340
High resolution limit [Å]	2.300	2.300
Rmerge	0.047	0.134
Number of reflections	24281
<I/σ(I)>	18.3
Completeness [%]	90.0	84
Redundancy	3.1	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	25% PEG 4000, 0.1M NH4CL, 0.1M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	HEPES
2	1	1	NH4CL
3	1	1	PEG 4000
4	1	2	PEG 4000