1QAG
Actin binding region of the dystrophin homologue utrophin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1998-01-21 |
| Detector | PRINCETON 2K |
| Wavelength(s) | 0.9000, 0.9795, 0.9809 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 150.145, 55.160, 80.420 |
| Unit cell angles | 90.00, 106.06, 90.00 |
Refinement procedure
| Resolution | 99.000 - 3.000 |
| R-factor | 0.198 * |
| Rwork | 0.198 |
| R-free | 0.25800 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXS |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.000 * | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.057 | 0.146 |
| Number of reflections | 12342 | |
| <I/σ(I)> | 8 | 5.1 |
| Completeness [%] | 96.7 | 97.2 |
| Redundancy | 2.7 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 4.7 * | 20 * | 0.1M Sodium Acetate pH 4.7, 2.0 M unbuffered sodium formate., pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Sodium Acetate | ||
| 2 | 1 | 1 | sodium formate |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 50 (mg/ml) | |
| 2 | 1 | reservoir | 2.0 (M) | ||
| 3 | 1 | reservoir | sodium acetate | 0.1 (M) |
