1QAD
Crystal Structure of the C-Terminal SH2 Domain of the P85 alpha Regulatory Subunit of Phosphoinositide 3-Kinase: An SH2 domain mimicking its own substrate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX7.2 |
| Synchrotron site | SRS |
| Beamline | PX7.2 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-06-05 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 38.680, 46.920, 62.980 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 23.440 - 1.800 |
| R-factor | 0.158 * |
| Rwork | 0.158 |
| R-free | 0.23600 |
| Starting model (for MR) | 1bfj |
| RMSD bond length | 0.008 * |
| RMSD bond angle | 2.100 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.440 | 1.850 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.044 * | 0.068 * |
| Total number of observations | 37237 * | |
| Number of reflections | 10947 | 677 * |
| <I/σ(I)> | 7.2 | 10.2 |
| Completeness [%] | 98.7 | 84.5 |
| Redundancy | 3.4 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 4.6 * | 277 | Eck, M.J., (1993) Nature, 362, 87. * |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | 1mM protein (~13mg/ml) | ||
| 2 | 1 | 2 | 50mM TRIS-HCL | ||
| 3 | 1 | 2 | 50mM NaCl | ||
| 4 | 1 | 2 | 1mM DTT |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG2000 | 25-35 (%) | can be replaced by PEG4000 or PEG8000 |
| 2 | 1 | reservoir | 50 (mM) | ||
| 3 | 1 | reservoir | sodium acetate | 100 (mM) | pH4.6 |
| 4 | 1 | drop | protein | 50 (mg/ml) |
