1QAC
CHANGE IN DIMERIZATION MODE BY REMOVAL OF A SINGLE UNSATISFIED POLAR RESIDUE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1998-11-29 |
| Detector | CUSTOM-MADE |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 35.300, 83.600, 42.800 |
| Unit cell angles | 90.00, 109.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.800 |
| Rwork | 0.193 |
| R-free | 0.22600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3LVE with GLU-38 and GLN-89 changed to ALA |
| RMSD bond length | 0.006 |
| RMSD bond angle | 26.000 * |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (0.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.056 | 0.172 |
| Number of reflections | 21776 | |
| <I/σ(I)> | 21.9 | 5.6 |
| Completeness [%] | 97.9 | |
| Redundancy | 8.6 | 9.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 277 | 18% PEG 8000, 0.2M AMMONIUM SULPHATE AT 4 DEG C, VAPOR DIFFUSION, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG8000 | 18 (%) | |
| 2 | 1 | reservoir | ammonium sulfate | 0.2 (M) |
