1Q9D
Fructose-1,6-bisphosphatase Complexed with a New Allosteric Site Inhibitor (I-State)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 120 |
| Detector technology | AREA DETECTOR |
| Collection date | 1999-06-16 |
| Detector | SIEMENS |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 58.860, 166.250, 80.270 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.350 |
| R-factor | 0.197 |
| Rwork | 0.191 |
| R-free | 0.24600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1eyj |
| RMSD bond length | 0.012 |
| RMSD bond angle | 24.100 * |
| Data reduction software | X-GEN |
| Data scaling software | X-GEN |
| Phasing software | X-PLOR |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.450 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.059 | |
| Total number of observations | 94949 * | |
| Number of reflections | 28849 | |
| Completeness [%] | 87.5 | 65.5 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 20 * | PEG 3350, Tris-malonate, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | KPi | 10 (mM) | pH7.4 |
| 3 | 1 | drop | 5 (mM) | ||
| 4 | 1 | drop | F6P | 5 (mM) | |
| 5 | 1 | drop | OC252 | 2 (mM) | |
| 6 | 1 | reservoir | Tris malonate | 2.5 (mM) | pH7.4 |
| 7 | 1 | reservoir | PEG3350 | 6 (%(w/v)) |
