1Q91

Crystal structure of human mitochondrial deoxyribonucleotidase in complex with the inhibitor DPB-T

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2002-01-30
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9393
Spacegroup name	P 43 21 2
Unit cell lengths	73.651, 73.651, 106.830
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.770 - 1.600
R-factor	0.186
Rwork	0.186
R-free	0.20600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	pdb code 1MH9
RMSD bond length	0.026
RMSD bond angle	2.200
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.0)

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.3	298	PEG 8000, potassium phosphate, pH 5.3, VAPOR DIFFUSION, HANGING DROP, temperature 298K