1Q90

Structure of the cytochrome b6f (plastohydroquinone : plastocyanin oxidoreductase) from Chlamydomonas reinhardtii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM30A
Synchrotron site	ESRF
Beamline	BM30A
Temperature [K]	100
Detector technology	CCD
Collection date	2002-09-15
Detector	MARRESEARCH
Wavelength(s)	1.00799
Spacegroup name	I 2 2 2
Unit cell lengths	102.454, 171.205, 351.009
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.000 * - 3.100
R-factor	0.222
Rwork	0.222
R-free	0.26000 *
Structure solution method	MIR
RMSD bond length	0.008
RMSD bond angle	1.320 *
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	MLPHARE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.000	3.270
High resolution limit [Å]	3.100	3.100
Rmerge	0.089 *	0.390 *
Number of reflections	56687
<I/σ(I)>	6.6
Completeness [%]	99.6	99.6
Redundancy	4.3 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8 *	277	Reservoir: 25% PEG-MME 350, 40 millimolar TRIS HCL PH 8, 40 millimolar nacl, 0.2 millimolar laurylmaltoside, 30% glycerol. Drop: 1.3 microliter protein + 0.7 microliter reservoir, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	Tris-HCl	40 (mM)	pH8
2	1	reservoir		40 (mM)
3	1	reservoir	DDM	0.2 (mM)
4	1	reservoir	glycerol	30 (%(w/v))
5	1	reservoir	PEG350	25 (%(w/v))
6	1	drop	protein	10 (mg/ml)