1Q8Z
The apoenzyme structure of the yeast SR protein kinase, Sky1p
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 94 |
| Detector technology | CCD |
| Collection date | 2002-06-15 |
| Detector | SBC-2 |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 72.990, 88.701, 135.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.350 |
| Rwork | 0.221 |
| R-free | 0.25900 |
| Starting model (for MR) | PDB code 1HOW |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.330 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.430 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.067 * | 0.636 * |
| Total number of observations | 198041 * | |
| Number of reflections | 34172 | |
| <I/σ(I)> | 10.3 | |
| Completeness [%] | 91.6 | 85.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 4.5 | 298 | Nolen, B., (2001) Nat.Struct.Biol., 8, 176. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | drop | ATP | 0.0066 (mM) | |
| 3 | 1 | drop | 0.0017 (mM) | ||
| 4 | 1 | reservoir | ammonium sulfate | 1.5 (M) | |
| 5 | 1 | reservoir | ethylene glycol | 15 (%(v/v)) | |
| 6 | 1 | reservoir | sodium acetate | 10 (mM) |
