1Q8M

Crystal structure of the human myeloid cell activating receptor TREM-1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Wavelength(s)	1.0376, 0.9641, 0.9790, 0.9791
Spacegroup name	P 1 21 1
Unit cell lengths	50.315, 128.595, 62.389
Unit cell angles	90.00, 101.69, 90.00

Refinement procedure

Resolution	46.000 - 2.600
Rwork	0.201
R-free	0.25100
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.670
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.000	2.700
High resolution limit [Å]	2.600	2.600
Rmerge	0.086 *	0.440 *
Number of reflections	23897 *	2369 *
<I/σ(I)>	23.5	4.2
Completeness [%]	98.6 *	97.7 *
Redundancy	4.2	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.7	298	Ammonium sulfate, MES, pH 5.7, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	2.1-2.4 (M)
2	1	reservoir	MES	100 (mM)	pH5.4-5.7
3	1	reservoir	sodium citrate