1Q8I
Crystal structure of ESCHERICHIA coli DNA Polymerase II
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 5ID-B |
Synchrotron site | APS |
Beamline | 5ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MARRESEARCH |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 92.383, 116.596, 82.430 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.000 |
R-factor | 0.19872 |
Rwork | 0.197 |
R-free | 0.23612 |
Structure solution method | MIRAS |
RMSD bond length | 0.017 |
RMSD bond angle | 1.636 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MLPHARE |
Refinement software | REFMAC (5.0) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 29.700 |
High resolution limit [Å] | 2.000 |
Number of reflections | 60657 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.3 | 295 | Citrate , PEG3K, DTT, EDTA, Glycerol, pH 5.3, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
1 | VAPOR DIFFUSION, HANGING DROP | 5.3 | 295 | Citrate , PEG3K, DTT, EDTA, Glycerol, pH 5.3, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
1 | VAPOR DIFFUSION, HANGING DROP | 5.3 | 295 | Citrate , PEG3K, DTT, EDTA, Glycerol, pH 5.3, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
1 | VAPOR DIFFUSION, HANGING DROP | 5.3 | 295 | Citrate , PEG3K, DTT, EDTA, Glycerol, pH 5.3, VAPOR DIFFUSION, HANGING DROP, temperature 295K |