1Q8F
Crystal Structure of the E.coli pyrimidine nucleoside hydrolase yeiK
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-05-04 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.933 |
| Spacegroup name | P 1 |
| Unit cell lengths | 44.808, 85.707, 90.678 |
| Unit cell angles | 112.95, 101.95, 85.92 |
Refinement procedure
| Resolution | 43.830 - 1.700 |
| R-factor | 0.15348 |
| Rwork | 0.153 |
| R-free | 0.16989 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mas |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.307 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.19) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 81.600 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 122468 | |
| <I/σ(I)> | 9.5 | 3 |
| Completeness [%] | 91.5 | 63.8 |
| Redundancy | 2 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295 | PEGMME 3350, methanol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
