1Q8C

A conserved hypothetical protein from Mycoplasma genitalium shows structural homology to NusB proteins

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 210
Wavelength(s)	0.9790, 0.9638, 1.0086
Spacegroup name	P 41
Unit cell lengths	41.401, 41.401, 99.008
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.990 * - 2.000
R-factor	0.25527
Rwork	0.254
R-free	0.27200 *
Structure solution method	MAD
RMSD bond length	0.013
RMSD bond angle	1.470 *
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.050
High resolution limit [Å]	2.000	2.000
Rmerge	0.061 *	0.680 *
Total number of observations	35519 *
Number of reflections	11273 *
Completeness [%]	94.7 *	99.8 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *		4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	22 (mg/ml)
2	1	reservoir	sodium iodide	0.2 (M)
3	1	reservoir	PEG3350	20 (%)