1Q89

Crystal structure of the C-domain of the T.vaginalis Inr binding protein, IBP39 (cubic crystal form)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	2001-12-10
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	I 2 3
Unit cell lengths	108.750, 108.750, 108.750
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	76.500 - 2.750
R-factor	0.197
Rwork	0.197
R-free	0.26400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1q88
RMSD bond length	0.008
RMSD bond angle	1.190 *
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	MOLREP
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	76.500	2.820
High resolution limit [Å]	2.750	2.750
Rmerge	0.074	0.246 *
Total number of observations	35034 *
Number of reflections	6272
Completeness [%]	94.0	89

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6.5	298	PEG 5000, ammonium sulphate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20-50 (mg/ml)
2	1	reservoir	ammonium sulfate	200 (mM)
3	1	reservoir	MES	50 (mM)	pH6.5
4	1	reservoir	PEG5000	25 (%)