1Q88

Crystal structure of the C-domain of the T.vaginalis Inr binding protein, IBP39 (monoclinic form)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	2000-12-10
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	C 1 2 1
Unit cell lengths	99.600, 76.400, 80.300
Unit cell angles	90.00, 93.00, 90.00

Refinement procedure

Resolution	49.700 * - 2.420
R-factor	0.19
Rwork	0.190
R-free	0.23400 *
Structure solution method	MIR
RMSD bond length	0.009
RMSD bond angle	1.500 *
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	CNS
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.700	2.500
High resolution limit [Å]	2.420	2.420
Rmerge	0.064 *	0.245 *
Total number of observations	62819 *
Number of reflections	18750
<I/σ(I)>	15.2
Completeness [%]	98.9	83

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6.5	298	PEG 5000, ammonium sulphate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20-50 (mg/ml)
2	1	reservoir	ammonium sulfate	200 (mM)
3	1	reservoir	MES	50 (mM)	pH6.5
4	1	reservoir	PEG5000	15 (%)
5	1	reservoir	sodium phosphate	1 (M)	pH7.0