1Q7Q
Cobalamin-dependent methionine synthase (1-566) from T. maritima (Oxidized, Orthorhombic)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 5ID-B |
| Synchrotron site | APS |
| Beamline | 5ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-03-12 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 92.583, 169.022, 174.276 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.100 |
| R-factor | 0.253 |
| Rwork | 0.221 |
| R-free | 0.24800 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 * |
| RMSD bond angle | 1.360 * |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.240 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.074 * | 0.263 * |
| Number of reflections | 45652 | |
| Completeness [%] | 89.4 * | 63.6 * |
| Redundancy | 2.6 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.7 | 4 * | propylene glycol, glycerol, PEG 8000, citrate, 1,2,3-heptanetriol, cadmium, pH 5.7, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | 10 (mM) | ||
| 2 | 1 | drop | 1,2,3-heptanetriol | 1.25 (%(w/v)) | |
| 3 | 1 | reservoir | 1,2-propanediol | 25 (%) | |
| 4 | 1 | reservoir | glycerol | 10 (%) | |
| 5 | 1 | reservoir | PEG3000 | 5 (%) | |
| 6 | 1 | reservoir | sodium citrate | 100 (mM) | pH5.30 |
