1Q7M
Cobalamin-dependent methionine synthase (MetH) from Thermotoga maritima (Oxidized, Monoclinic)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 5ID-B |
| Synchrotron site | APS |
| Beamline | 5ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-03-12 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.700, 84.480, 125.670 |
| Unit cell angles | 90.00, 100.87, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.100 |
| R-factor | 0.2 |
| Rwork | 0.198 |
| R-free | 0.23800 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 * |
| RMSD bond angle | 1.346 * |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.200 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.054 * | 0.222 * |
| Number of reflections | 58895 * | |
| Completeness [%] | 100.0 * | 100 * |
| Redundancy | 9.2 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.7 * | 4 * | propylene glycol, glycerol, phosphate, citrate, PEG 8000, pH 5.2, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | 10 (mM) | ||
| 2 | 1 | drop | 1,2,3-heptanetriol | 1.25 (%(w/v)) | |
| 3 | 1 | reservoir | 1,2-propanediol | 25 (%) | |
| 4 | 1 | reservoir | glycerol | 10 (%) | |
| 5 | 1 | reservoir | PEG3000 | 5 (%) | |
| 6 | 1 | reservoir | sodium citrate | 100 (mM) | pH5.30 |
