1Q7F
Brain Tumor NHL domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2001-03-01 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 45.770, 94.580, 130.500 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.000 - 1.950 |
R-factor | 0.2002 |
Rwork | 0.200 |
R-free | 0.25230 |
Structure solution method | MAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.358 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.000 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.063 | 0.126 |
Total number of observations | 250484 * | |
Number of reflections | 42132 * | |
<I/σ(I)> | 7.3 | |
Completeness [%] | 84.1 * | 68.7 |
Redundancy | 5.94 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 * | 20 * | 55% amm PO4 5% MPD, pH 8.2, VAPOR DIFFUSION, HANGING DROP, temperature 20K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 25 (mg/ml) | |
2 | 1 | drop | Bis-Tris | 20 (mM) | |
3 | 1 | drop | 50 (mM) | ||
4 | 1 | drop | dithiothreitol | 1 (mM) | pH6 |
5 | 1 | reservoir | di-basic ammonium phosphate | 53 (%) | |
6 | 1 | reservoir | MPD | 4 (%) |