1Q79

CRYSTAL STRUCTURE OF MAMMALIAN POLY(A) POLYMERASE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12C
Synchrotron site	NSLS
Beamline	X12C
Temperature [K]	100
Detector technology	CCD
Collection date	2001-08-04
Detector	BRANDEIS
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	57.640, 63.070, 181.340
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.790 - 2.150
R-factor	0.205
Rwork	0.205
R-free	0.23500
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.006
RMSD bond angle	1.200
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.250
High resolution limit [Å]	2.150	2.150
Rmerge	0.049
Number of reflections	34205
<I/σ(I)>	22.6	4.2
Completeness [%]	92.7	70.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	297	PEG8000, ammonium sulfate, MES buffer, calcium chloride, manganese chloride, pH 6.00, VAPOR DIFFUSION, HANGING DROP, temperature 297K