1Q6U
Crystal structure of FkpA from Escherichia coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2001-04-09 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.934 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 115.180, 132.750, 41.780 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.450 |
| R-factor | 0.21391 |
| Rwork | 0.211 |
| R-free | 0.27700 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fd9 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.700 * |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.580 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.081 * | 0.394 * |
| Number of reflections | 12110 | 1695 * |
| <I/σ(I)> | 7.5 | 1.8 |
| Completeness [%] | 99.2 * | 99.2 |
| Redundancy | 6.2 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 4.5 * | 17 * | MES, jeffamine M600, CsCl, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 296K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG2000 | 30 (%(v/v)) | |
| 2 | 1 | reservoir | sodium acetate | 0.1 (M) | pH4.5 |
| 3 | 1 | drop | protein | 3.1 (mg/ml) |
