1Q6C

Crystal Structure of Soybean Beta-Amylase Complexed with Maltose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1996-01-10
Detector	RIGAKU RAXIS IIC
Wavelength(s)	1.5418
Spacegroup name	P 31 2 1
Unit cell lengths	86.158, 86.158, 144.608
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 * - 1.860
R-factor	0.174
Rwork	0.174
R-free	0.21200 *
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.290 *
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.070	2.000
High resolution limit [Å]	1.860	1.860
Rmerge	0.096 *	0.247 *
Total number of observations	122982 *
Number of reflections	39604	3719 *
Completeness [%]	86.1	76.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.4	4 *	ammonium sulfate, pH 5.4, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	24 (mg/ml)
2	1	drop	sodium acetate	0.1 (M)	pH5.4
3	1	reservoir	ammonium sulfate	48 (%)
4	1	reservoir	sodium acetate	0.1 (M)
5	1	reservoir	EDTA	1 (mM)
6	1	reservoir	2-mercaptoethanol	18 (mM)	pH5.4