1Q5X

Structure of OF RRAA (MENG), a protein inhibitor of RNA processing

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	103
Detector technology	IMAGE PLATE
Collection date	2001-07-09
Detector	RIGAKU RAXIS IV
Spacegroup name	C 1 2 1
Unit cell lengths	94.454, 93.945, 67.949
Unit cell angles	90.00, 132.72, 90.00

Refinement procedure

Resolution	20.000 - 2.000
R-factor	0.228
Rwork	0.226
R-free	0.27000 *
Structure solution method	SIRAS
RMSD bond length	0.006
RMSD bond angle	1.246 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.071	0.328
Number of reflections	28089
<I/σ(I)>	13.4
Completeness [%]	95.5	71.8
Redundancy	2.9	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.2	298	AMMONIUM PHOSPHATE, SODIUM CITRATE, pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium phosphate	0.8 (M)
2	1	reservoir	sodium citrate	0.1 (M)	pH5.2