1Q5R

The Rhodococcus 20S proteasome with unprocessed pro-peptides

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	110
Detector technology	CCD
Collection date	2000-10-01
Detector	ADSC QUANTUM 4
Wavelength(s)	1
Spacegroup name	C 2 2 21
Unit cell lengths	152.094, 212.365, 250.213
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 * - 3.100
R-factor	0.229
Rwork	0.229
R-free	0.24600
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	23.000 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	3.100
Rmerge	0.084
Number of reflections	66375
Completeness [%]	88.7 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7 *	298	14% PEG 6000, 50mM sodium citrate, pH 5.4, VAPOR DIFFUSION, SITTING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	8 (mg/ml)
2	1	drop	Tris-HCl	10 (mM)	pH7.0
3	1	reservoir	PEG6000	14 (%(w/v))
4	1	reservoir	sodium citrate	50 (mM)	pH5.4