1Q4Q

Crystal structure of a DIAP1-Dronc complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	100
Wavelength(s)	1.1
Spacegroup name	P 65
Unit cell lengths	128.900, 128.900, 183.695
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.100
R-factor	0.203
Rwork	0.199
R-free	0.24600
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009 *
RMSD bond angle	1.531 *
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	99.000	2.180
High resolution limit [Å]	2.100	2.100
Rmerge	0.057 *	0.173 *
Total number of observations	582663 *
Number of reflections	94061
Completeness [%]	94.2	82 *
Redundancy	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.8	293	Citrate, PEG 4000, ammonium sulfate, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	sodium citrate	100 (mM)	pH5.8
3	1	reservoir	PEG4000	15 (%(w/v))
4	1	reservoir	ammonium sulfate	120 (mM)