1Q3L
Chromodomain Of HP1 Complexed With Histone H3 Tail Containing monomethyllysine 9.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-01-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.92080 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 33.809, 76.828, 75.625 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.350 - 1.640 |
| R-factor | 0.22315 |
| Rwork | 0.222 |
| R-free | 0.24091 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1kna |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.898 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.19) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.350 | 1.700 |
| High resolution limit [Å] | 1.640 | 1.640 |
| Number of reflections | 10752 | |
| <I/σ(I)> | 52.9 | 20.5 |
| Completeness [%] | 0.9 | 0.988 |
| Redundancy | 7.37 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.1 | 283 | Ammonium Sulfate, MES, pH 6.1, VAPOR DIFFUSION, SITTING DROP, temperature 283K |
