1Q34
Crystal structures of two UBC (E2) enzymes of the ubiquitin-conjugating system in Caenorhabditis elegans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-11-10 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.072 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 100.922, 37.584, 103.881 |
| Unit cell angles | 90.00, 117.58, 90.00 |
Refinement procedure
| Resolution | 19.740 - 2.900 |
| R-factor | 0.233 |
| Rwork | 0.233 |
| R-free | 0.26600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2aak |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.500 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.870 |
| High resolution limit [Å] | 2.770 | 2.770 |
| Rmerge | 0.039 | |
| Number of reflections | 17357 | |
| <I/σ(I)> | 16.2 | |
| Completeness [%] | 95.8 | 83.1 |
| Redundancy | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.6 | 295 | PEG 8000, Calcium Acetate, Glycerol, Glycine, pH 9.6, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
