1Q31
Crystal Structure of the Tobacco Etch Virus Protease C151A mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-09-13 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.933 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 146.587, 41.544, 96.063 |
| Unit cell angles | 90.00, 108.95, 90.00 |
Refinement procedure
| Resolution | 36.580 - 2.700 |
| R-factor | 0.24 |
| Rwork | 0.240 |
| R-free | 0.30200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lvb |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.720 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 91.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 13436 | |
| Completeness [%] | 88.0 | 93.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | lithium sulphate monohydrate, Tris HCL, PEG 4000, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
