1Q29

Hammerhead Ribozyme with 5'-5' G-G linkage: Conformational change experiment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Spacegroup name	P 31 2 1
Unit cell lengths	66.689, 66.689, 140.528
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.800 - 3.000
R-factor	0.23 *
Rwork	0.242
R-free	0.26000 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	NDB entry urx057
RMSD bond length	0.012 *
RMSD bond angle	2.518 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	CNS
Refinement software	REFMAC (5.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000 *
High resolution limit [Å]	2.990 *
Rmerge	0.089 *	0.300 *
Total number of observations	7686 *
Completeness [%]	98.6 *	92.8 *
Redundancy	5.3 *	5.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5 *	16 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	ribozyme	1 (mM)
2	1	drop	sodium acetate	50 (mM)	pH5.0
3	1	drop		1.8 (M)
4	1	reservoir	sodium acetate	50 (mM)	pH5.0
5	1	reservoir		1.8 (M)
6	1	reservoir	EDTA	1.0 (mM)