1Q1S

Mouse Importin alpha- phosphorylated SV40 CN peptide complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2000-04-14
Detector	MARRESEARCH
Wavelength(s)	0.97
Spacegroup name	P 21 21 21
Unit cell lengths	78.827, 89.746, 99.707
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 * - 2.300
R-factor	0.198
Rwork	0.198
R-free	0.22100
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	1ial
RMSD bond length	0.008
RMSD bond angle	19.300 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	99.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.059	0.234
Total number of observations	272363 *
Number of reflections	32147
<I/σ(I)>		5.3
Completeness [%]	99.4	95.8
Redundancy	8.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6.5	293	Sodium Citrate, DTT, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	peptide	1.7 (mg/ml)
2	1	drop	sodium citrate	0.7-0.8 (M)
3	1	reservoir	HEPES	100 (mM)	pH6.0-6.5
4	1	reservoir	dithiothreitol	10 (mM)