1Q14

Structure and autoregulation of the yeast Hst2 homolog of Sir2

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2003-08-13
Detector	ADSC QUANTUM 4
Wavelength(s)	0.900
Spacegroup name	H 3 2
Unit cell lengths	103.554, 103.554, 177.346
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 2.500
R-factor	0.239
Rwork	0.219
R-free	0.25100
Structure solution method	MAD
RMSD bond length	0.006 *
RMSD bond angle	1.270 *
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.590
High resolution limit [Å]	2.500	2.500
Rmerge	0.057	0.220
Number of reflections	12776 *
<I/σ(I)>	34.8	8.9
Completeness [%]	98.6	99.9
Redundancy	14.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	PEg4K, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	4 (mg/ml)
2	1	reservoir	ammonium sulfate	1.8-1.9 (M)
3	1	reservoir	HEPES	100 (mM)	pH7.5
4	1	reservoir	PEG400	2 (%(v/v))