1Q14
Structure and autoregulation of the yeast Hst2 homolog of Sir2
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-08-13 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.900 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 103.554, 103.554, 177.346 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.500 |
| R-factor | 0.239 |
| Rwork | 0.219 |
| R-free | 0.25100 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 * |
| RMSD bond angle | 1.270 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.057 | 0.220 |
| Number of reflections | 12776 * | |
| <I/σ(I)> | 34.8 | 8.9 |
| Completeness [%] | 98.6 | 99.9 |
| Redundancy | 14.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | PEg4K, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 4 (mg/ml) | |
| 2 | 1 | reservoir | ammonium sulfate | 1.8-1.9 (M) | |
| 3 | 1 | reservoir | HEPES | 100 (mM) | pH7.5 |
| 4 | 1 | reservoir | PEG400 | 2 (%(v/v)) |
