1Q0Y

Anti-Morphine Antibody 9B1 Complexed with Morphine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	2002-10-16
Detector	RIGAKU RAXIS IV
Spacegroup name	C 1 2 1
Unit cell lengths	56.118, 60.024, 115.263
Unit cell angles	90.00, 92.39, 90.00

Refinement procedure

Resolution	33.000 - 2.000
R-factor	0.194
Rwork	0.194
R-free	0.23900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	15C8 (CONSTANT DOMAIN) + PDB ENTRY 1E4X (VARIABLE DOMAIN)
RMSD bond length	0.006
RMSD bond angle	1.340
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.076
Number of reflections	25462
<I/σ(I)>	14.7	2.2
Completeness [%]	97.8	94.7
Redundancy	2.8	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		4.6	298	22% PEG MME 2000, 0.2M ammonium sulfate, 0.1M sodium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K, pH 4.60