1Q0U

Crystal Structure of the BstDEAD N-terminal Domain

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL1-5
Synchrotron site	SSRL
Beamline	BL1-5
Temperature [K]	100
Detector technology	CCD
Collection date	2002-06-12
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9789, 0.9792, 0.9252
Spacegroup name	P 64
Unit cell lengths	113.463, 113.463, 65.338
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	25.000 - 1.850
R-factor	0.204
Rwork	0.201
R-free	0.28600 *
Structure solution method	MAD
RMSD bond length	0.011
RMSD bond angle	2.300 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SHARP
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	1.900
High resolution limit [Å]	1.850	1.850
Number of reflections	40958
<I/σ(I)>	6.6	1.9
Completeness [%]	98.8	98.8
Redundancy	8.2	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	10.5	298	PEG 1550, CAPS, pH 10.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	CAPS	100 (mM)	pH10.5
2	1	reservoir	PEG1550	30 (%(w/v))
3	1	drop	protein	57 (mg/ml)