1Q0O
CRYSTAL STRUCTURE OF HOMOPROTOCATECHUATE 2,3-DIOXYGENASE FROM BREVIBACTERIUM FUSCUM (FULL LENGTH PROTEIN)
Replaces: 1F1YExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | SIEMENS |
| Temperature [K] | 291 |
| Detector technology | AREA DETECTOR |
| Collection date | 1999-01-01 |
| Detector | SIEMENS HI-STAR |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 118.900, 118.900, 110.300 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| Rwork | 0.161 |
| R-free | 0.20800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.760 |
| Data reduction software | X-GEN |
| Data scaling software | X-GEN |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.370 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.089 | 0.323 |
| Number of reflections | 36551 | |
| <I/σ(I)> | 6.7 | 1 |
| Completeness [%] | 89.0 | 42.3 |
| Redundancy | 4.8 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch | 7.5 | 291 | 1.5 M ammonium sulfate, 100 mM Mops, pH 7.5, Batch, temperature 291K |
