1Q07
Crystal structure of the Au(I) form of E. coli CueR, a copper efflux regulator
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 5ID-B |
| Synchrotron site | APS |
| Beamline | 5ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-09-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0358 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 59.265, 66.323, 82.467 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.310 - 2.500 |
| R-factor | 0.209 |
| Rwork | 0.209 |
| R-free | 0.25300 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.100 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | SHARP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.000 | 2.560 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.057 | 0.287 |
| Number of reflections | 11501 | |
| <I/σ(I)> | 12.6 | 3.2 |
| Completeness [%] | 98.3 | 96.1 |
| Redundancy | 6.7 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | sodium citrate, sodium cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
