1PZX
Hypothetical protein APC36103 from Bacillus stearothermophilus: a lipid binding protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-06-23 |
| Detector | SBC-3 |
| Wavelength(s) | 0.9783 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 65.980, 187.390, 53.290 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.540 - 2.000 |
| R-factor | 0.197 |
| Rwork | 0.197 |
| R-free | 0.25200 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.300 |
| Data reduction software | d*TREK |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.095 | 0.452 |
| Number of reflections | 44031 | |
| <I/σ(I)> | 26.45 | 3.15 |
| Completeness [%] | 96.3 | 92.4 |
| Redundancy | 9.63 | 10.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289 | pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
