1PZV
Crystal structures of two UBC (E2) enzymes of the ubiquitin-conjugating system in Caenorhabditis elegans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-10-10 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.105 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.474, 52.184, 68.882 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.820 - 2.520 |
| R-factor | 0.241 |
| Rwork | 0.241 |
| R-free | 0.28900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ucz |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.500 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.520 | 2.520 |
| Rmerge | 0.054 | 0.336 |
| Number of reflections | 6215 | |
| <I/σ(I)> | 16 | |
| Completeness [%] | 99.6 | 99.6 |
| Redundancy | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.4 | 295 | PEG 5000 MME, CAPSO, glycerol, potassium thiocyanate, pH 9.4, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
