1PZS
Crystal Structure of a Cu-Zn Superoxide Dismutase from Mycobacterium tuberculosis at 1.63 resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID13 |
| Synchrotron site | ESRF |
| Beamline | ID13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-11-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9755 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 70.040, 58.420, 51.390 |
| Unit cell angles | 90.00, 126.99, 90.00 |
Refinement procedure
| Resolution | 27.950 - 1.630 |
| R-factor | 0.15233 |
| Rwork | 0.150 |
| R-free | 0.19027 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1JCV.pdb |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.356 |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.950 | 1.800 |
| High resolution limit [Å] | 1.634 | 1.634 |
| Rmerge | 0.067 | 0.273 |
| Number of reflections | 20248 | |
| <I/σ(I)> | 13.54 | 5.17 |
| Completeness [%] | 96.0 | 87.9 |
| Redundancy | 3.62 | 3.27 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | PEG 4000, Ammonium sulfate, zinc chloride, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 20K |
