1PZF

T.gondii LDH1 ternary complex with APAD+ and oxalate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2002-03-15
Detector	ADSC QUANTUM 4
Wavelength(s)	1.03
Spacegroup name	P 1 21 1
Unit cell lengths	68.065, 124.976, 86.686
Unit cell angles	90.00, 106.09, 90.00

Refinement procedure

Resolution	30.000 - 2.200
R-factor	0.1775
Rwork	0.177
R-free	0.21760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1pzh
RMSD bond length	0.009
RMSD bond angle	1.410 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASING (FROM ISOMORPHOUS STRUCTURE)
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.240
High resolution limit [Å]	2.200	2.200
Rmerge	0.121	0.386
Total number of observations	224085 *
Number of reflections	70313
<I/σ(I)>	9.2
Completeness [%]	99.1	91.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	4.5	293	0.9 M ammonium sulfate, 100 mM acetate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	12 (mg/ml)
2	1	drop	cosubstrate	10 (mM)
3	1	drop	oxalate	5 (mM)	or oxamate
4	1	reservoir	ammonium sulfate	0.85-0.90 (M)
5	1	reservoir	acetate	100 (mM)	pH4.5