1PZD

Structural Identification of a conserved appendage domain in the carboxyl-terminus of the COPI gamma-subunit.

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Wavelength(s)	0.92
Spacegroup name	P 21 21 21
Unit cell lengths	49.570, 89.560, 98.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.190 - 2.310
R-factor	0.199
Rwork	0.199
R-free	0.23600
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	25.100 *
Data reduction software	MOSFLM
Data scaling software	SCALEPACK
Phasing software	SnB
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.000	2.440
High resolution limit [Å]	2.300	2.300
Number of reflections	19621
<I/σ(I)>	6.3
Completeness [%]	89.7	87.5
Redundancy	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5 *	20 *	100mM sodium citrate and 1.5-1.7M lithium chloride, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 18K
1	VAPOR DIFFUSION, SITTING DROP	7.5 *	20 *	100mM sodium citrate and 1.5-1.7M lithium chloride, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 18K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	drop	Tris	20 (mM)	pH7.5
3	1	reservoir		1.4-1.7 (M)
4	1	reservoir	sodium citrate	100 (mM)	pH6.0