1PZ7

Modulation of agrin function by alternative splicing and Ca2+ binding

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X31
Synchrotron site	EMBL/DESY, Hamburg
Beamline	X31
Temperature [K]	100
Wavelength(s)	0.97834
Spacegroup name	C 1 2 1
Unit cell lengths	101.329, 57.540, 74.621
Unit cell angles	90.00, 127.10, 90.00

Refinement procedure

Resolution	20.000 - 1.421
R-factor	0.22116
Rwork	0.220
R-free	0.24017
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	1.326
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	SHARP
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall
Low resolution limit [Å]	24.400
High resolution limit [Å]	1.420
Number of reflections	63594
<I/σ(I)>	8.2
Completeness [%]	98.7
Redundancy	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	276	peg 8000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 276K