1PZ3

Crystal structure of a family 51 (GH51) alpha-L-arabinofuranosidase from Geobacillus stearothermophilus T6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	2002-11-09
Detector	MARRESEARCH
Wavelength(s)	1.54178
Spacegroup name	H 3
Unit cell lengths	179.430, 179.430, 100.231
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 1.750
Rwork	0.171
R-free	0.20400
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014 *
RMSD bond angle	1490.000 *
Data scaling software	SCALEPACK
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.790
High resolution limit [Å]	1.750	1.750
Rmerge	0.047 *	0.395 *
Number of reflections	119711
Completeness [%]	99.7	99.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	8	298	18 % PEG 3350, 0.2 M NH4F, 100 mM Tris/HCl, 5% 2-Propanol, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG3350	23 (%(w/v))
2	1	reservoir		0.2 (M)
3	1	reservoir	2-propanol	5 (%(v/v))
4	1	reservoir	Tris-HCl	0.1 (M)	pH8.0