1PYX
GSK-3 Beta complexed with AMP-PNP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-11-06 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 82.691, 85.209, 178.138 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 * - 2.400 |
| Rwork | 0.206 |
| R-free | 0.23300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1h8f |
| RMSD bond length | 0.010 |
| RMSD bond angle | 23.200 * |
| Data scaling software | SCALEPACK |
| Refinement software | CNX (2002) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.900 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.076 * | 0.518 * |
| Total number of observations | 206766 * | |
| Number of reflections | 50028 * | |
| <I/σ(I)> | 16 | 3.1 |
| Completeness [%] | 98.9 | 100 |
| Redundancy | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.2 * | 20 * | PEG 3350 monodisperse, Glycerol, Magnesium Chloride, Hepes, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K, pH 7.00 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 12 (mg/ml) | |
| 2 | 1 | drop | glycerol | 10 (%) | |
| 3 | 1 | drop | 185 (mM) | ||
| 4 | 1 | drop | HEPES | 100 (mM) | pH7.2 |
| 5 | 1 | reservoir | PEG3350 monodisperse | 16-24 (%(w/v)) | |
| 6 | 1 | reservoir | glycerol | 5 (%) | |
| 7 | 1 | reservoir | 20 (mM) | ||
| 8 | 1 | reservoir | HEPES | 100 (mM) | pH7.0 |
